Journal cover Journal topic
Magnetic Resonance An interactive open-access publication of the Groupement AMPERE
Journal topic
Discussion papers
https://doi.org/10.5194/mr-2020-5
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-2020-5
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Submitted as: research article 21 Feb 2020

Submitted as: research article | 21 Feb 2020

Review status
This preprint is currently under review for the journal MR.

Optimising broadband pulses for DEER depends on concentration and distance range of interest

Andreas Scherer, Sonja Tischlik, Sabrina Weickert, Valentin Wittmann, and Malte Drescher Andreas Scherer et al.
  • Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany

Abstract. EPR distance determination in the nanometre region has become an important tool for studying the structure and interaction of macromolecules. Arbitrary waveform generators (AWGs), which have recently become commercially available for EPR spectrometers, have the potential to increase the sensitivity of the most common technique double electron-electron resonance (DEER, also called PELDOR), as they allow the generation of broadband pulses. There are several families of broadband pulses, which are different in general pulse shape and the parameters that define them. Here, we compare the most common broadband pulses. When broadband pulses lead to a larger modulation depth they also increase the background decay of the DEER trace. Depending on the dipolar evolution time this can significantly increase the noise level towards the end of the form factor and limit the potential increase of the modulation to noise ratio (MNR). We found asymmetric hyperbolic secant (HS{1,6}) pulses to perform best for short DEER traces leading to a MNR improvement of up to 86 % compared to rectangular pulses. For longer traces we found symmetric hyperbolic secant (HS{1,1}) pulses to perform best, however, the increase compared to rectangular pulses goes down to 43 %.

Andreas Scherer et al.

Interactive discussion

Status: open (until 08 Apr 2020)
Status: open (until 08 Apr 2020)
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
[Subscribe to comment alert] Printer-friendly Version - Printer-friendly version Supplement - Supplement

Andreas Scherer et al.

Data sets

Raw data for 'Optimising broadband pulses for DEER depends on concentration and distance range of interest' A. Scherer, S. Tischlik, S. Weickert, V. Wittmann, and M. Drescher https://doi.org/10.5281/zenodo.3660241

Andreas Scherer et al.

Viewed

Total article views: 174 (including HTML, PDF, and XML)
HTML PDF XML Total Supplement BibTeX EndNote
140 30 4 174 16 4 3
  • HTML: 140
  • PDF: 30
  • XML: 4
  • Total: 174
  • Supplement: 16
  • BibTeX: 4
  • EndNote: 3
Views and downloads (calculated since 21 Feb 2020)
Cumulative views and downloads (calculated since 21 Feb 2020)

Viewed (geographical distribution)

Total article views: 122 (including HTML, PDF, and XML) Thereof 122 with geography defined and 0 with unknown origin.
Country # Views %
  • 1
1
 
 
 
 

Cited

Saved

No saved metrics found.

Discussed

No discussed metrics found.
Latest update: 03 Apr 2020
Publications Copernicus
Download
Short summary
The determination of distance distributions in the nanometre range is an important application of pulsed electron paramagnetic resonance spectroscopy. However, low sensitivity is often a major challenge. In this paper, we compare several broadband shaped pulses and compare their performance to classical rectangular pulses in order to increase the sensitivity of double electron-electron resonance on a commercial setup. We show that improvements in sensitivity of up to 86 % are possible.
The determination of distance distributions in the nanometre range is an important application...
Citation